LDHD ELISA 试剂盒
Quick Overview for LDHD ELISA 试剂盒 (ABIN2043869)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.56 ng/mL
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灵敏度
- < 0.59 ng/mL
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Lactate Dehydrogenase D (LDHD) ELISA Kit
LDHD 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 pmol/mL - 10 pmol/mL Plasma, Serum
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD 适用: 小鼠 Colorimetric Sandwich ELISA 4.69 mU/mL - 300 mU/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Lactate Dehydrogenase D (LDHD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase D (LDHD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase D (LDHD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase D (LDHD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lactate Dehydrogenase D (LDHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- LDHD (Lactate Dehydrogenase D (LDHD))
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途径
- Ribonucleoside Biosynthetic Process
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