KRT40 ELISA 试剂盒
This 人 KRT40 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 KRT40.
Quick Overview for KRT40 ELISA 试剂盒 (ABIN2043857)
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KRT40
(Keratin 40 (KRT40))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.064 ng/mL
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Keratin 40 (KRT40) ELISA Kit
KRT40 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
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板类型
- Pre-coated
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实验流程
- Keratin 40 (KRT40) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 40 (KRT40). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 40 (KRT40). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 40 (KRT40), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 40 (KRT40) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- KRT40 (Keratin 40 (KRT40))
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