Keratin 33A ELISA 试剂盒
The 人 Keratin 33A ELISA Kit (ABIN2043855) is a Colorimetric ELISA Kit designed to quantify 人 Keratin 33A.
产品编号 ABIN2043855
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for Keratin 33A ELISA 试剂盒 (ABIN2043855)
抗原
See all Keratin 33A (KRT33A) products
Keratin 33A (KRT33A)
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
78.12-5000 pg/mL
应用范围
ELISA
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最低检测浓度
- 78.12 pg/mL
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灵敏度
- < 29 pg/mL
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Keratin 33A (KRT33A) ELISA Kit
KRT33A 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Keratin 33A (KRT33A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 33A (KRT33A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 33A (KRT33A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 33A (KRT33A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 33A (KRT33A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Keratin 33A (KRT33A)
抗原 See all Keratin 33A (KRT33A) products
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