JAM2 ELISA 试剂盒
This 人 JAM2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 JAM2.
Quick Overview for JAM2 ELISA 试剂盒 (ABIN2043831)
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JAM2
(Junctional Adhesion Molecule 2 (JAM2))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.2-2000 pg/mL
应用范围
ELISA
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最低检测浓度
- 31.2 pg/mL
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品牌
- BioAssay™
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Analytical Method
- Quantitative
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灵敏度
- 13.3 pg/mL
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组件
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A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A (green), 1x120 µL
E: Detection Reagent B (red), 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL
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Junctional Adhesion Molecule 2 (JAM2) ELISA Kit
JAM2 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- The Human Junctional Adhesion Molecule 2 (JAM2) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of JAM2 in human serum, plasma, tissue homogenates and other biological fluids.The Microtiter Strip provided in this kit has been pre-coated with an antibody specific to JAM2. Standards or samples are then added to the appropriate Microtiter Strip wells with a biotin-conjugated antibody specific to JAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of JAM2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- JAM2 (Junctional Adhesion Molecule 2 (JAM2))
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