IL2RG ELISA 试剂盒
This 人 IL2RG ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IL2RG.
Quick Overview for IL2RG ELISA 试剂盒 (ABIN2043807)
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IL2RG
(Interleukin 2 Receptor, gamma (IL2RG))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.054 ng/mL
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Interleukin 2 Receptor, gamma (IL2RG) ELISA Kit
IL2RG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Interleukin 2 Receptor Gamma (IL2Rg) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Gamma (IL2Rg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 Receptor Gamma (IL2Rg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 Receptor Gamma (IL2Rg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 2 Receptor Gamma (IL2Rg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL2RG (Interleukin 2 Receptor, gamma (IL2RG))
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别名
- Interleukin 2 Receptor gamma (IL2Rg)
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途径
- JAK/STAT Signaling, Growth Factor Binding
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