IL12B ELISA 试剂盒
Quick Overview for IL12B ELISA 试剂盒 (ABIN2043801)
抗原
See all IL12B ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 7.81 pg/mL
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灵敏度
- < 2.8 pg/mL
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Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 马 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 小鼠 AA 23-335 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 绵羊 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 山羊 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitHigh Sensitivity IL12B 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Interleukin 12B (IL12B) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B (IL12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12B (IL12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12B (IL12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 12B (IL12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL12B (Interleukin 12 beta (IL12B))
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别名
- Interleukin 12B (IL12B)
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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