IL12RB1 ELISA 试剂盒
This 人 IL12RB1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IL12RB1.
Quick Overview for IL12RB1 ELISA 试剂盒 (ABIN2043799)
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IL12RB1
(Interleukin 12 Receptor beta 1 (IL12RB1))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.071 ng/mL
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Interleukin 12 Receptor beta 1 (IL12RB1) ELISA Kit
IL12RB1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Interleukin 12 Receptor Beta 1 (IL12Rb1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 Receptor Beta 1 (IL12Rb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 12 Receptor Beta 1 (IL12Rb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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