IRS3 ELISA 试剂盒
This 大鼠 IRS3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 IRS3.
Quick Overview for IRS3 ELISA 试剂盒 (ABIN2043765)
抗原
See all IRS3 products
IRS3
(Insulin Receptor Substrate 3 (IRS3))
适用
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.62-40 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.62 ng/mL
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灵敏度
- < 0.227 ng/mL
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板类型
- Pre-coated
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实验流程
- Insulin Receptor Substrate 3 (IRS3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 3 (IRS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Receptor Substrate 3 (IRS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Receptor Substrate 3 (IRS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Receptor Substrate 3 (IRS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IRS3 (Insulin Receptor Substrate 3 (IRS3))
抗原 See all IRS3 products
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