HTRA1 ELISA 试剂盒
Quick Overview for HTRA1 ELISA 试剂盒 (ABIN2043717)
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See all HTRA1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.25 pg/mL
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灵敏度
- < 14.3 pg/mL
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组件
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A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL
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HtrA Serine Peptidase 1 (HTRA1) ELISA Kit
HTRA1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
HtrA Serine Peptidase 1 (HTRA1) ELISA KitHTRA1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
HtrA Serine Peptidase 1 (HTRA1) ELISA KitHTRA1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
HtrA Serine Peptidase 1 (HTRA1) ELISA KitHTRA1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- The Human HtrA Serine Peptidase (HTRA1) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HTRA1 in human serum, plasma and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTRA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HTRA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTRA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HTRA1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- HTRA1 (HtrA Serine Peptidase 1 (HTRA1))
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途径
- Growth Factor Binding
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