HINT2 ELISA 试剂盒
This 小鼠 HINT2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 HINT2.
Quick Overview for HINT2 ELISA 试剂盒 (ABIN2043701)
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HINT2
(Histidine Triad Nucleotide Binding Protein 2 (HINT2))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.061 ng/mL
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Histidine Triad Nucleotide Binding Protein 2 (HINT2) ELISA Kit
HINT2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Histidine Triad Nucleotide Binding Protein 2 (HINT2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Triad Nucleotide Binding Protein 2 (HINT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Triad Nucleotide Binding Protein 2 (HINT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Triad Nucleotide Binding Protein 2 (HINT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Histidine Triad Nucleotide Binding Protein 2 (HINT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- HINT2 (Histidine Triad Nucleotide Binding Protein 2 (HINT2))
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