ChT ELISA 试剂盒
This 大鼠 ChT ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 ChT.
Quick Overview for ChT ELISA 试剂盒 (ABIN2043643)
抗原
See all ChT ELISA试剂盒
ChT
(High Affinity Choline Transporter (ChT))
适用
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
78.12-5000 pg/mL
应用范围
ELISA
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最低检测浓度
- 78.12 pg/mL
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灵敏度
- < 28 pg/mL
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板类型
- Pre-coated
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实验流程
- High Affinity Choline Transporter (ChT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Affinity Choline Transporter (ChT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Affinity Choline Transporter (ChT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Affinity Choline Transporter (ChT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of High Affinity Choline Transporter (ChT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- ChT (High Affinity Choline Transporter (ChT))
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