HSPB3 ELISA 试剂盒
This 人 HSPB3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 HSPB3.
Quick Overview for HSPB3 ELISA 试剂盒 (ABIN2043619)
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HSPB3
(Heat Shock 27kDa Protein 3 (HSPB3))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.78-50 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.78 ng/mL
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灵敏度
- < 0.29 ng/mL
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Heat Shock 27kDa Protein 3 (HSPB3) ELISA Kit
HSPB3 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Heat Shock Protein Beta 3 (HSPb3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 3 (HSPb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 3 (HSPb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 3 (HSPb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock Protein Beta 3 (HSPb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- HSPB3 (Heat Shock 27kDa Protein 3 (HSPB3))
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别名
- Heat Shock Protein beta 3 (HSPb3)
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