HSPA1B ELISA 试剂盒
Quick Overview for HSPA1B ELISA 试剂盒 (ABIN2043613)
抗原
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.56 ng/mL
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灵敏度
- < 0.53 ng/mL
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Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit
HSPA1B 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock 70kDa Protein 1B (HSPA1B) ELISA KitHSPA1B 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Heat Shock 70kDa Protein 1B (HSPA1B) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock 70kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))
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