GUCY1B3 ELISA 试剂盒
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Quick Overview for GUCY1B3 ELISA 试剂盒 (ABIN2043607)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.059 ng/mL
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Guanylate Cyclase 1, Soluble, beta 3 (GUCY1B3) ELISA Kit
GUCY1B3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Guanylate Cyclase 1, Soluble, beta 3 (GUCY1B3) ELISA KitGUCY1B3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Guanylate Cyclase 1 Beta 3 (GUCY1b3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase 1 Beta 3 (GUCY1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Guanylate Cyclase 1 Beta 3 (GUCY1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- GUCY1B3 (Guanylate Cyclase 1, Soluble, beta 3 (GUCY1B3))
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别名
- Guanylate Cyclase 1 beta 3 (GUCY1b3)
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途径
- Transition Metal Ion Homeostasis
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