GADD45G ELISA 试剂盒
Quick Overview for GADD45G ELISA 试剂盒 (ABIN2043601)
抗原
See all GADD45G ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.62 ng/mL
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灵敏度
- < 0.225 ng/mL
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Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G) ELISA Kit
GADD45G 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G) ELISA KitGADD45G 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))
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别名
- Growth Arrest And DNA Damage Inducible Protein gamma (GADD45g)
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途径
- Cell Division Cycle
抗原 See all GADD45G ELISA试剂盒
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