GLDC ELISA 试剂盒
This 人 GLDC ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 GLDC.
Quick Overview for GLDC ELISA 试剂盒 (ABIN2043575)
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GLDC
(Glycine Dehydrogenase (GLDC))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.132 ng/mL
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Glycine Dehydrogenase (GLDC) ELISA Kit
GLDC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glycine Dehydrogenase (GLDC) ELISA KitGLDC 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Glycine Dehydrogenase (GLDC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycine Dehydrogenase (GLDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glycine Dehydrogenase (GLDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- GLDC (Glycine Dehydrogenase (GLDC))
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