GLB1 ELISA 试剂盒
Quick Overview for GLB1 ELISA 试剂盒 (ABIN2043529)
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See all GLB1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.116 ng/mL
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Galactosidase, beta 1 (GLB1) ELISA Kit
GLB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 mIU/mL - 10 mIU/mL Plasma, Serum, Tissue Homogenate
Galactosidase, beta 1 (GLB1) ELISA KitGLB1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 mIU/mL - 10 mIU/mL Plasma, Serum, Tissue Homogenate
Galactosidase, beta 1 (GLB1) ELISA KitGLB1 适用: 人 Colorimetric Sandwich ELISA 3.13 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum
Galactosidase, beta 1 (GLB1) ELISA KitGLB1 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Galactosidase, beta 1 (GLB1) ELISA KitGLB1 适用: 人 Colorimetric Sandwich ELISA 7.8 μIU/mL - 500 μIU/mL Plasma, Serum, Tissue Homogenate, Urine
SensoLyte® ONPG b-Galactosidase Assay KitEAA Colorimetric
SensoLyte® MUG b-Galactosidase Assay KitEAA Fluorometric
SensoLyte® FDG b-Galactosidase Assay KitEAA Fluorometric
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板类型
- Pre-coated
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实验流程
- Galactosidase Beta (GLb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactosidase Beta (GLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactosidase Beta (GLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactosidase Beta (GLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Galactosidase Beta (GLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- GLB1 (Galactosidase, beta 1 (GLB1))
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别名
- Galactosidase beta (GLb)
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途径
- Glycosaminoglycan Metabolic Process
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