FAK ELISA 试剂盒
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Quick Overview for FAK ELISA 试剂盒 (ABIN2043497)
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See all FAK (PTK2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.112 ng/mL
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PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA Kit
PTK2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 大鼠 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 小鼠 Colorimetric Sandwich ELISA 0.781-50 ng/mL Plasma, Serum
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板类型
- Pre-coated
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实验流程
- Focal Adhesion Kinase (FAK) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Focal Adhesion Kinase (FAK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Focal Adhesion Kinase (FAK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Focal Adhesion Kinase (FAK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Focal Adhesion Kinase (FAK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
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别名
- Focal Adhesion Kinase (FAK)
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途径
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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