FBLIM1 ELISA 试剂盒
This 大鼠 FBLIM1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 FBLIM1.
Quick Overview for FBLIM1 ELISA 试剂盒 (ABIN2043485)
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FBLIM1
(Filamin Binding LIM Protein 1 (FBLIM1))
适用
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.058 ng/mL
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板类型
- Pre-coated
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实验流程
- Filamin Binding LIM Protein 1 (FBLIM1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin Binding LIM Protein 1 (FBLIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin Binding LIM Protein 1 (FBLIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin Binding LIM Protein 1 (FBLIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Filamin Binding LIM Protein 1 (FBLIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))
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