FAM135B ELISA 试剂盒
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Quick Overview for FAM135B ELISA 试剂盒 (ABIN2043433)
抗原
See all FAM135B products适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.057 ng/mL
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板类型
- Pre-coated
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实验流程
- Family With Sequence Similarity 135, Member B (FAM135B) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 135, Member B (FAM135B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Family With Sequence Similarity 135, Member B (FAM135B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))
抗原 See all FAM135B products
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