EPHX4 ELISA 试剂盒
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Quick Overview for EPHX4 ELISA 试剂盒 (ABIN2043409)
抗原
See all EPHX4 products适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 62.5 pg/mL
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灵敏度
- < 27.2 pg/mL
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Epoxide Hydrolase 4 (EPHX4) ELISA Kit
EPHX4 适用: 人 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Epoxide Hydrolase 4 (EPHX4) ELISA KitEPHX4 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epoxide Hydrolase 4 (EPHX4) ELISA KitEPHX4 适用: 人 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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板类型
- Pre-coated
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实验流程
- Epoxide Hydrolase 4 (EPHX4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epoxide Hydrolase 4 (EPHX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epoxide Hydrolase 4 (EPHX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epoxide Hydrolase 4 (EPHX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Epoxide Hydrolase 4 (EPHX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- EPHX4 (Epoxide Hydrolase 4 (EPHX4))
抗原 See all EPHX4 products
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