ETFB ELISA 试剂盒
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Quick Overview for ETFB ELISA 试剂盒 (ABIN2043393)
抗原
See all ETFB ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.112 ng/mL
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板类型
- Pre-coated
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实验流程
- Electron Transfer Flavoprotein Beta Polypeptide (ETFb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Beta Polypeptide (ETFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Electron Transfer Flavoprotein Beta Polypeptide (ETFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))
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别名
- Electron Transfer Flavoprotein beta Polypeptide (ETFb)
抗原 See all ETFB ELISA试剂盒
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