CELA2A ELISA 试剂盒
Quick Overview for CELA2A ELISA 试剂盒 (ABIN2043389)
抗原
See all CELA2A ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.25 pg/mL
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品牌
- BioAssay™
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灵敏度
- < 12.8 pg/mL
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Chymotrypsin-Like Elastase Family, Member 2A (CELA2A) ELISA Kit
CELA2A 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chymotrypsin-Like Elastase Family, Member 2A (CELA2A) ELISA KitCELA2A 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Elastase 2A (ELA2A) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 2A (ELA2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 2A (ELA2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 2A (ELA2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Elastase 2A (ELA2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CELA2A (Chymotrypsin-Like Elastase Family, Member 2A (CELA2A))
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别名
- Elastase 2A (ELA2A)
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