AKR1C2 ELISA 试剂盒
This 人 AKR1C2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 AKR1C2.
Quick Overview for AKR1C2 ELISA 试剂盒 (ABIN2043343)
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AKR1C2
(Aldo-keto Reductase Family 1, Member C2 (AKR1C2))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.061 ng/mL
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Aldo-keto Reductase Family 1, Member C2 (AKR1C2) ELISA Kit
AKR1C2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Dihydrodiol Dehydrogenase 2 (DDH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrodiol Dehydrogenase 2 (DDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dihydrodiol Dehydrogenase 2 (DDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))
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别名
- Dihydrodiol Dehydrogenase 2 (DDH2)
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途径
- Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process
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