DSE ELISA 试剂盒
This 人 DSE ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 DSE.
Quick Overview for DSE ELISA 试剂盒 (ABIN2043329)
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DSE
(Dermatan Sulfate Epimerase (DSE))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.114 ng/mL
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Dermatan Sulfate Epimerase (DSE) ELISA Kit
DSE 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Dermatan Sulfate Epimerase (DSE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatan Sulfate Epimerase (DSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatan Sulfate Epimerase (DSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatan Sulfate Epimerase (DSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dermatan Sulfate Epimerase (DSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- DSE (Dermatan Sulfate Epimerase (DSE))
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途径
- Glycosaminoglycan Metabolic Process
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