beta 2 Defensin ELISA 试剂盒
Quick Overview for beta 2 Defensin ELISA 试剂盒 (ABIN2043311)
抗原
See all beta 2 Defensin (BD-2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 62.5 pg/mL
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灵敏度
- < 22.6 pg/mL
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Defensin beta 2 (BD-2) ELISA Kit
BD-2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Defensin beta 2 (BD-2) ELISA KitBD-2 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Defensin beta 2 (BD-2) ELISA KitBD-2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Defensin beta 2 (BD-2) ELISA KitBD-2 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Defensin beta 2 (BD-2) ELISA KitBD-2 适用: 小鼠 Colorimetric Competition ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Defensin beta 2 (BD-2) ELISA KitBD-2 适用: 犬 Colorimetric Competition ELISA 100-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Defensin Beta 2 (DEFb2) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 2 (DEFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 2 (DEFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 2 (DEFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 2 (DEFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))
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别名
- Defensin beta 2 (DEFb2)
抗原 See all beta 2 Defensin (BD-2) ELISA试剂盒
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