DEFB126 ELISA 试剂盒
This 人 DEFB126 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 DEFB126.
Quick Overview for DEFB126 ELISA 试剂盒 (ABIN2043307)
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DEFB126
(Defensin, beta 126 (DEFB126))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
7.81-500 pg/mL
应用范围
ELISA
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最低检测浓度
- 7.81 pg/mL
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灵敏度
- < 3.4 pg/mL
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Defensin, beta 126 (DEFB126) ELISA Kit
DEFB126 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Defensin Beta 126 (DEFb126) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 126 (DEFb126). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 126 (DEFb126). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 126 (DEFb126), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 126 (DEFb126) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- DEFB126 (Defensin, beta 126 (DEFB126))
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别名
- Defensin beta 126 (DEFb126)
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