CLOCK ELISA 试剂盒
Quick Overview for CLOCK ELISA 试剂盒 (ABIN2043169)
抗原
See all CLOCK ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.109 ng/mL
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Clock Homolog (Mouse) (CLOCK) ELISA Kit
CLOCK 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Clock Homolog (Mouse) (CLOCK) ELISA KitCLOCK 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Clock Homolog (CLOCK) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clock Homolog (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Clock Homolog (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clock Homolog (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Clock Homolog (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CLOCK (Clock Homolog (Mouse) (CLOCK))
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别名
- Clock Homolog (CLOCK)
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途径
- Regulation of Lipid Metabolism by PPARalpha, Photoperiodism
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