CGB ELISA 试剂盒
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Quick Overview for CGB ELISA 试剂盒 (ABIN2043149)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6.25 ng/mL
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灵敏度
- < 2.75 ng/mL
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Chorionic Gonadotropin, beta Polypeptide (CGB) ELISA Kit
CGB 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chorionic Gonadotropin, beta Polypeptide (CGB) ELISA KitCGB 适用: 猴 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Chorionic Gonadotropin, beta Polypeptide (CGB) ELISA KitCGB 适用: 大鼠 Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Chorionic Gonadotropin, beta Polypeptide (CGB) ELISA KitCGB 适用: 人 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Chorionic Gonadotropin Beta Polypeptide (CGb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chorionic Gonadotropin Beta Polypeptide (CGb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CGB (Chorionic Gonadotropin, beta Polypeptide (CGB))
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别名
- Chorionic Gonadotropin beta Polypeptide (CGb)
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