CHRNB1 ELISA 试剂盒
This 大鼠 CHRNB1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 CHRNB1.
Quick Overview for CHRNB1 ELISA 试剂盒 (ABIN2043147)
抗原
See all CHRNB1 ELISA试剂盒
CHRNB1
(Nicotinic Acetylcholine Receptor beta (CHRNB1))
适用
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
78.12-5000 pg/mL
应用范围
ELISA
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最低检测浓度
- 78.12 pg/mL
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灵敏度
- < 31 pg/mL
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板类型
- Pre-coated
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实验流程
- Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))
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别名
- Cholinergic Receptor, Nicotinic, beta 1 (CHRNb1)
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途径
- Synaptic Membrane
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