Chitotriosidase 1 ELISA 试剂盒
Quick Overview for Chitotriosidase 1 ELISA 试剂盒 (ABIN2043135)
抗原
See all Chitotriosidase 1 (CHIT1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.134 ng/mL
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Chitotriosidase (CHIT1) ELISA Kit
CHIT1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chitotriosidase (CHIT1) ELISA KitCHIT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Chitinase 1 (CHIT1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase 1 (CHIT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chitinase 1 (CHIT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chitinase 1 (CHIT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chitinase 1 (CHIT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
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别名
- Chitinase 1 (CHIT1)
抗原 See all Chitotriosidase 1 (CHIT1) ELISA试剂盒
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