CENPE ELISA 试剂盒
This 人 CENPE ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 CENPE.
Quick Overview for CENPE ELISA 试剂盒 (ABIN2043113)
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CENPE
(Centromere Protein E, 312kDa (CENPE))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.124 ng/mL
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Centromere Protein E, 312kDa (CENPE) ELISA Kit
CENPE 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Centromere Protein E (CENPE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Centromere Protein E (CENPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Centromere Protein E (CENPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Centromere Protein E (CENPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Centromere Protein E (CENPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CENPE (Centromere Protein E, 312kDa (CENPE))
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别名
- Centromere Protein E (CENPE)
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途径
- M Phase, Maintenance of Protein Location
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