BTK ELISA 试剂盒
Quick Overview for BTK ELISA 试剂盒 (ABIN2043023)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.057 ng/mL
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Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA Kit
BTK 适用: 人 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Bruton Agammaglobulinemia tyrosine Kinase (BTK) ELISA KitBTK 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Bruton'S Tyrosine Kinase (Btk) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bruton'S Tyrosine Kinase (Btk). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bruton'S Tyrosine Kinase (Btk). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bruton'S Tyrosine Kinase (Btk), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bruton'S Tyrosine Kinase (Btk) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))
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别名
- Bruton'S Tyrosine Kinase (Btk)
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途径
- Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling
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