BHMT ELISA 试剂盒
This 人 BHMT ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 BHMT.
Quick Overview for BHMT ELISA 试剂盒 (ABIN2043009)
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BHMT
(Betaine--Homocysteine S-Methyltransferase (BHMT))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.124 ng/mL
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Betaine--Homocysteine S-Methyltransferase (BHMT) ELISA Kit
BHMT 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Betaine Homocysteine Methyltransferase (BHMT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))
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别名
- Betaine Homocysteine Methyltransferase (BHMT)
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途径
- Methionine Biosynthetic Process
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