AVPR2 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 小鼠 AVPR2.
产品编号 ABIN2042939
发货至:
中国
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Contact Info
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中国
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北京 101111
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Quick Overview for AVPR2 ELISA 试剂盒 (ABIN2042939)
抗原
See all AVPR2 products
AVPR2
(Arginine Vasopressin Receptor 2 (AVPR2))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.118 ng/mL
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板类型
- Pre-coated
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实验流程
- Arginine Vasopressin Receptor 2 (AVPR2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginine Vasopressin Receptor 2 (AVPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arginine Vasopressin Receptor 2 (AVPR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- AVPR2 (Arginine Vasopressin Receptor 2 (AVPR2))
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途径
- cAMP Metabolic Process
抗原 See all AVPR2 products
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