APOA5 ELISA 试剂盒
Quick Overview for APOA5 ELISA 试剂盒 (ABIN2042923)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 3.12 pg/mL
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灵敏度
- < 1.45 pg/mL
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Apolipoprotein A-V (APOA5) ELISA Kit
APOA5 适用: 人 Colorimetric Competition ELISA 2.5 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitWide Range APOA5 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 适用: 大鼠 Colorimetric Competition ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Apolipoprotein A5 (APOA5) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A5 (APOA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A5 (APOA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A5 (APOA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A5 (APOA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- APOA5 (Apolipoprotein A-V (APOA5))
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别名
- Apolipoprotein A5 (APOA5)
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途径
- Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism
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