Anti-Sperm Antibody (AsAb) ELISA 试剂盒
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北京 101111
Quick Overview for Anti-Sperm Antibody (AsAb) ELISA 试剂盒 (ABIN2042911)
抗原
适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.56 ng/mL
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灵敏度
- < 0.59 ng/mL
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板类型
- Pre-coated
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实验流程
- Anti-Sperm Antibody (AsAb) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Sperm Antibody (AsAb) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Sperm Antibody (AsAb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Anti-Sperm Antibody (AsAb)
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物质类
- Antibody
抗原
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