MBL Ab ELISA 试剂盒
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Quick Overview for MBL Ab ELISA 试剂盒 (ABIN2042897)
抗原
See all MBL Ab products适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6.25 ng/mL
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灵敏度
- < 2.77 ng/mL
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板类型
- Pre-coated
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实验流程
- Anti-Mannose Binding Lectin Antibody (Anti-MBL) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Mannose Binding Lectin Antibody (Anti-MBL) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mannose Binding Lectin Antibody (Anti-MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MBL Ab (Anti-Mannose Binding Lectin Antibody (MBL Ab))
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物质类
- Antibody, Antibody
抗原 See all MBL Ab products
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