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GAD Ab ELISA 试剂盒

人 GAD Ab ELISA Kit, Colorimetric is an assay for quantification of 人 GAD Ab.
产品编号 ABIN2042887
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GAD Ab ELISA 试剂盒 (ABIN2042887)

抗原

See all GAD Ab products
GAD Ab (Glutamic Acid Decarboxylase Antibody (GAD Ab))

适用

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检测方法

Colorimetric

实验类型

Competition ELISA

检测范围

1.56-100 ng/mL

应用范围

ELISA
  • 最低检测浓度

    1.56 ng/mL

    Analytical Method

    Quantitative

    灵敏度

    < 0.65 ng/mL

    组件

    A: Microtiter Plate, 96 wells, Pre-coated, ready to use
    B: Standard, 2x1vial
    C: Standard Diluent, 1x20 mL
    D: Detection Reagent A, 1x120 µL
    E: Assay Diluent A, 1x10 mL
    F: TMB Substrate, 1x0 mL
    G: Stop Solution, 1x0 mL
    H: Wash Buffer, 30X, 1x20 mL
  • 板类型

    Pre-coated

    实验流程

    The Human Anti-Glutamic Acid Decarboxylase Antibodies (Anti-GAD) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-GAD in human serum, plasma and other biological fluids.The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-GAD will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-GAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all GAD Ab products

    GAD Ab (Glutamic Acid Decarboxylase Antibody (GAD Ab))

    别名

    Anti-Glutamic Acid Decarboxylase Antibodies (Anti-GAD)

    物质类

    Antibody, Antibody
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