GAD Ab ELISA 试剂盒
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北京 101111
Quick Overview for GAD Ab ELISA 试剂盒 (ABIN2042887)
抗原
See all GAD Ab products适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.56 ng/mL
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Analytical Method
- Quantitative
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灵敏度
- < 0.65 ng/mL
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组件
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A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Assay Diluent A, 1x10 mL
F: TMB Substrate, 1x0 mL
G: Stop Solution, 1x0 mL
H: Wash Buffer, 30X, 1x20 mL
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板类型
- Pre-coated
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实验流程
- The Human Anti-Glutamic Acid Decarboxylase Antibodies (Anti-GAD) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-GAD in human serum, plasma and other biological fluids.The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-GAD will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-GAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- GAD Ab (Glutamic Acid Decarboxylase Antibody (GAD Ab))
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别名
- Anti-Glutamic Acid Decarboxylase Antibodies (Anti-GAD)
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物质类
- Antibody, Antibody
抗原 See all GAD Ab products
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