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alpha Fetoprotein ELISA 试剂盒

This 猴 alpha Fetoprotein ELISA Kit is a Colorimetric ELISA Kit designed to quantify 猴 alpha Fetoprotein.
产品编号 ABIN2042821
发货至: 中国

Quick Overview for alpha Fetoprotein ELISA 试剂盒 (ABIN2042821)

抗原

See all alpha Fetoprotein (AFP) ELISA试剂盒
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.31-20 ng/mL

应用范围

ELISA
  • 最低检测浓度

    0.31 ng/mL

    灵敏度

    < 0.118 ng/mL
  • 板类型

    Pre-coated

    实验流程

    Alpha-Fetoprotein (aFP) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-Fetoprotein (aFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all alpha Fetoprotein (AFP) ELISA试剂盒

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    途径

    C21-Steroid Hormone Metabolic Process
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