GRK2 ELISA 试剂盒
Quick Overview for GRK2 ELISA 试剂盒 (ABIN2042791)
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See all GRK2 (ADRBK1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.105 ng/mL
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Adrenergic, Beta, Receptor Kinase 1 (ADRBK1) ELISA Kit
ADRBK1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenergic, Beta, Receptor Kinase 1 (ADRBK1) ELISA KitADRBK1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenergic, Beta, Receptor Kinase 1 (ADRBK1) ELISA KitADRBK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Adrenergic, Beta, Receptor Kinase 1 (ADRBK1) ELISA KitADRBK1 适用: 人 Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Adrenergic Receptor Beta Kinase 1 (ADRbK1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Beta Kinase 1 (ADRbK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Beta Kinase 1 (ADRbK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Beta Kinase 1 (ADRbK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adrenergic Receptor Beta Kinase 1 (ADRbK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))
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别名
- Adrenergic Receptor beta Kinase 1 (ADRbK1)
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途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
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