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APMAP ELISA 试剂盒

This 人 APMAP ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 APMAP.
产品编号 ABIN2042789
发货至: 中国

Quick Overview for APMAP ELISA 试剂盒 (ABIN2042789)

抗原

See all APMAP ELISA试剂盒
APMAP (Adipocyte Plasma Membrane Associated Protein (APMAP))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.15-10 ng/mL

应用范围

ELISA
  • 最低检测浓度

    0.15 ng/mL

    灵敏度

    < 0.066 ng/mL
  • 板类型

    Pre-coated

    实验流程

    Adipocyte Plasma Membrane Associated Protein (APMAP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adipocyte Plasma Membrane Associated Protein (APMAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adipocyte Plasma Membrane Associated Protein (APMAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adipocyte Plasma Membrane Associated Protein (APMAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adipocyte Plasma Membrane Associated Protein (APMAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all APMAP ELISA试剂盒

    APMAP (Adipocyte Plasma Membrane Associated Protein (APMAP))
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