ADCY10 ELISA 试剂盒
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Quick Overview for ADCY10 ELISA 试剂盒 (ABIN2042777)
抗原
See all ADCY10 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.125 ng/mL
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Adenylate Cyclase 10 (Soluble) (ADCY10) ELISA Kit
ADCY10 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase 10 (Soluble) (ADCY10) ELISA KitADCY10 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Adenylate Cyclase 10, Soluble (ADCY10) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 10, Soluble (ADCY10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 10, Soluble (ADCY10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))
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别名
- Adenylate Cyclase 10, Soluble (ADCY10)
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途径
- cAMP Metabolic Process
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