ATF4 ELISA 试剂盒
Quick Overview for ATF4 ELISA 试剂盒 (ABIN2042769)
抗原
See all ATF4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 78.12 pg/mL
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灵敏度
- < 28 pg/mL
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Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) ELISA Kit
ATF4 适用: 大鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) ELISA KitATF4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) ELISA KitATF4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) ELISA KitATF4 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Activating Transcription Factor 4 (ATF4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Transcription Factor 4 (ATF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Transcription Factor 4 (ATF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Transcription Factor 4 (ATF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Activating Transcription Factor 4 (ATF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))
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别名
- Activating Transcription Factor 4 (ATF4)
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途径
- Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
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