Acetyl-CoA Carboxylase alpha ELISA 试剂盒
Quick Overview for Acetyl-CoA Carboxylase alpha ELISA 试剂盒 (ABIN2042755)
抗原
See all Acetyl-CoA Carboxylase alpha (ACACA) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 3.12 ng/mL
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灵敏度
- < 1.34 ng/mL
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Acetyl-CoA Carboxylase alpha (ACACA) ELISA Kit
ACACA 适用: 人 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Acetyl-CoA Carboxylase alpha (ACACA) ELISA KitACACA 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Acetyl-CoA Carboxylase alpha (ACACA) ELISA KitACACA 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Acetyl-CoA Carboxylase alpha (ACACA) ELISA KitACACA 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Acetyl Coenzyme A Carboxylase Alpha (ACACa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Carboxylase Alpha (ACACa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Carboxylase Alpha (ACACa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Acetyl-CoA Carboxylase alpha (ACACA)
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别名
- Acetyl Coenzyme A Carboxylase alpha (ACACa)
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途径
- AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect
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