ADAM33 ELISA 试剂盒
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Quick Overview for ADAM33 ELISA 试剂盒 (ABIN2042743)
抗原
See all ADAM33 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.060 ng/mL
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ADAM Metallopeptidase Domain 33 (ADAM33) ELISA Kit
ADAM33 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 33 (ADAM33) ELISA KitADAM33 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Saliva, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 33 (ADAM33) ELISA KitADAM33 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
ADAM Metallopeptidase Domain 33 (ADAM33) ELISA KitADAM33 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- A Disintegrin And Metalloprotease 33 (ADAM33) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 33 (ADAM33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of A Disintegrin And Metalloprotease 33 (ADAM33) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- ADAM33 (ADAM Metallopeptidase Domain 33 (ADAM33))
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别名
- A Disintegrin And Metalloprotease 33 (ADAM33)
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