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VCAM1 ELISA 试剂盒

This 人 VCAM1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 VCAM1.
产品编号 ABIN2042718
发货至: 中国

Quick Overview for VCAM1 ELISA 试剂盒 (ABIN2042718)

抗原

See all VCAM1 ELISA试剂盒
VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

6-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-VCAM-1, Human
    Lyophilized recombinant VCAM-1, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human VCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.1 Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.2 Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.3 The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.1 The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74kD. As a result of glycosylation, the molecular mass of 90-95kD is revealed by SDS-PAGE.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all VCAM1 ELISA试剂盒

    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

    别名

    VCAM-1

    基因ID

    7412

    UniProt

    P19320

    途径

    Carbohydrate Homeostasis
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