VCAM1 ELISA 试剂盒
Quick Overview for VCAM1 ELISA 试剂盒 (ABIN2042717)
抗原
See all VCAM1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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96-well plate pre-coated with anti-VCAM-1, Mouse
Lyophilized recombinant VCAM-1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse VCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9kD. As a result of glycosylation, the molecular mass of 120kD is revealed by SDS-PAGE.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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别名
- VCAM-1
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基因ID
- 22329
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UniProt
- P29533
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途径
- Carbohydrate Homeostasis
抗原 See all VCAM1 ELISA试剂盒
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