PLAUR ELISA 试剂盒
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Quick Overview for PLAUR ELISA 试剂盒 (ABIN2042716)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 62.5 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 4 pg/mL
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组件
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96-well plate pre-coated with anti-uPAR, Human
Lyophilized recombinant uPAR, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human uPAR in cell culture supernates, serum, plasma (heparin, EDTA) and urine.The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1 The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with the molecular mass of 31kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))
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别名
- uPAR
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基因ID
- 5329
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UniProt
- Q03405
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途径
- Inositol Metabolic Process
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