Thrombopoietin ELISA 试剂盒
Quick Overview for Thrombopoietin ELISA 试剂盒 (ABIN2042708)
抗原
See all Thrombopoietin (THPO) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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96-well plate pre-coated with anti-TPO, Mouse
Lyophilized recombinant TPO, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse TPO in cell culture supernates, serum and plasma (heparin, EDTA).Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels.1 Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. 2,3 The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35kD. As a result of glycosylation, the molecular mass is 75kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Thrombopoietin (THPO)
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别名
- Thrombopoietin (TPO)
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基因ID
- 21832
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UniProt
- P40226
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途径
- JAK/STAT Signaling, Hormone Activity
抗原 See all Thrombopoietin (THPO) ELISA试剂盒
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